Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation
Fig 5
Relative mRNA expression levels of HAS2, PTGS2, PTX3 and TNFAIP6 in mature cumulus-oocyte complexes (COCs).
Expression levels are shown as relative quantities and were analysed using real-time polymerase chain reaction. β-Actin was used to normalise gene expression, and mature COCs without 17β-estradiol (E2) were used as calibrators. (a), (b), (c) and (d) represent the relative levels of HAS2, PTGS2, PTX3, and TNFAIP6 mRNA in mature COCs, respectively. Significant differences between different groups are indicated by different letters on the bars (P < 0.05).