All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
Fig 7
Shha expression pattern from early embryonic to late larval stages.
Whole mount in situ hybridization was performed in embryos and larvae at different developmental stages: (0.8 dpf: A—C; 1 dpf: D—F; 4 dpf: G—I; 10 dpf: J—L; 24 dpf: M–O, enlarged in M´- O´) and illustrated in lateral (A, D, G, J, M, M´), dorsal (B, E, H, K, N, N´) and frontal views (C, F, I, L, O, O´). Brass wild type line embryos (A—L) and casper line larvae (M–O, M´- O´) have been used. For staining, all embryos and larvae were incubated in BM purple for 22 hours.