All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
Fig 4
Dlx2a expression pattern from early embryonic to late larval stages is not affected by PTU treatment.
Whole mount in situ hybridization was performed in embryos and larvae at different developmental stages (1 dpf: A—C; 4 dpf: D—F; 10 dpf: G—I; 24 dpf: J–L, enlarged in J´- L´) and illustrated in lateral (A, D, G, J, J´), dorsal (B, E, H, K, K´) and frontal views (C, F, I, L, L´). Brass wild type line embryos (A—I) and casper line larvae (J–L, J´- L´) have been used. Embryos at 4 dpf (D—F) and 10 dpf (G—I) were treated with PTU to suppress pigmentation, embryos at 1dpf (A—C) and 24 dpf (J–L, J´- L´) were untreated. For staining, all embryos and larvae were incubated in BM purple for 22 hours.