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All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish

Fig 3

Eomesa expression in zebrafish embryos and young larval stages.

Whole mount in situ hybridization was performed in embryos and larvae at different developmental stages (3 dpf: A—C; 5 dpf: D—F; 7 dpf: G—I; 9 dpf J–L; 10 dpf: M–O) and illustrated in lateral (A, D, G, J, M), dorsal (B, E, H, K, N) and frontal views (C, F, I, L, O). For staining, all embryos and larvae were incubated in BM purple for 22 hours.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0237167.g003