Alternative isoforms of KDM2A and KDM2B lysine demethylases negatively regulate canonical Wnt signaling
Fig 6
Both the canonical and the demethylase domain deficient isoforms of KDM2A and KDM2B interact with TCF7L1.
The myc-tagged TCF7L1 was overexpressed in HEK293T cells together with the FLAG-tagged KDM2A/B isoforms and the proteins immunoprecipitated with anti-FLAG antibody were analyzed by western blot. All the four FLAG-tagged KDM2A/B isoforms co-immunoprecipitated with TCF7L1. The input corresponds to 5% of the whole cell extract that was used for each immunoprecipitation reaction.