Alternative isoforms of KDM2A and KDM2B lysine demethylases negatively regulate canonical Wnt signaling
Fig 4
KDM2A-SF and KDM2B-SF repress the axin 2 promoter.
A. The axin 2 promoter luciferase reporter constructs contain either a 1kb axin 2 intron 1 region (pAXIN2-1kb) or a 2.5kb axin 2 intron 1 region (pAXIN2-2.5kb). B. KDM2A-LF, KDM2A-SF and KDM2B-SF all repressed the pAXIN2-2.5kb reporter, whereas KDM2B-LF did not. The activity of the pAXIN2-2.5kb reporter is expressed as the fold change ratio between the activity of pAXIN2-2.5kb and that of pAXIN2-1kb. Similar pattern was observed when the results were expressed as the fold change between the activity of pAXIN2-2.5kb and the empty luciferase plasmid. (*p < 0.05; **p < 0.01).