Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae
Fig 2
Effect of PHO13 deletion on xylose fermentation by two xylose-metabolizing strains.
(A) The XYL123 strain expressing the xylose oxidoreductase pathway and (B) the XI-XYL3 strain expressing the xylose isomerase pathway were compared to their corresponding pho13Δ mutants (C and D, respectively). (E, F) Volumetric growth rates (g/L-h) and product yields (g/g) of the xylose fermentations. Fermentations were performed in YP medium containing 40 g/L xylose under oxygen-limited conditions (80 rpm), with a low initial cell density (0.5 g DCW/L). Asterisks denote statistically significant differences (Student's t-test, p < 0.05). n. d.; Not detected.