Local stimulation of osteocytes using a magnetically actuated oscillating beam
Fig 5
Osteocyte calcium response under magnetic actuator stimulation.
a) The shear stress map obtained using Eq 6 with velocities obtained from finite element simulations is overlaid with the locations of all responding cells from multiple trials that are coded with the time it takes to respond. The dotted line shows the typical viewing window in experiments and the range of the beam oscillation is also overlaid on the map. b) A representative image obtained of the Fura-2 AM stained cells along with the beam is shown which corresponds to the dotted lines on the shear stress map. Scale bar = 50 μm. c) A plot of the cell response time vs distance from the tip of the beam with a linear regression performed is shown, showing an increase in response time for cells further away from the stimulated region. A total of 3 trials and 39 cells were recorded, with an r2 value of 0.59 for the linear regression.