Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage
Fig 4
Co-localization of the G8 and R&D BAI1 mAbs in the skin and eyes.
Tissue sections of human tattooed skin (A-C), human anterior lens tissue (D-F), rabbit eyes (G-I) and rat retina (J-L) were double labeled with the G8 (red) and BAI1 (green) mAbs. Nuclei were stained with Hoechst dye (blue). Unmerged images are shown in A, B, D, E and the insets in G-I and K and L. Overlap of red and green appear yellow in triple merged images in C, F, G-I, K and L. A merge of fluorescence and DIC is shown in the inset of C. The G8 and BAI1 mAbs labeled the same cells in the skin, human lens, rabbit lens (G), ciliary body (H) and cornea (I), and the inner plexiform and inner nuclear layers of the mouse retina (K and L). Minimal background fluorescence was observed in the anti-IgM and anti-IgG secondary antibody controls for the skin (M), human lens tissue (N), rabbit lens (O) and mouse retina P and Q. Bar = 9 μM in A-I and K-Q and 54 μM in J.