Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage
Fig 1
Identification of the target of the G8 mAb.
The G8 mAb was used to screen clones of HEK-239T cells expressing a single human membrane protein. G8 binding was detected by flow cytometry. A. Binding values were normalized and transformed to give a single numerical value for binding of the G8 mAb against each target protein (normalized target binding). Non-specific fluorescence was determined to be any value below three standard deviations above noise. G8 binding above background occurred with the clone expressing BAI1. B. Validation of binding of the G8 mAb to BAI1 or vector alone was carried out with HEK-293T cells transfected with the plasmid construct expressing target using a serial dilution of mAb. C. Binding of different concentrations of the G8 mAb was calculated as the signal to background ratio of fluorescence from the BAI1 expressing clone to empty vector clone.