Antidepressive effect of an inward rectifier K+ channel blocker peptide, tertiapin-RQ
Fig 4
Chemically synthesized TPN-RQ (100 μL, 16.5 μM) was fractionated on a cation exchange column, monitoring A230 nm (A). A peak was found with a retention time of 17.5 min on the declining baseline (arrow). Then, the peptides, which were mixed with mouse serum, were analyzed (B and C). The incubation with serum did not affect the peak height or retention time. The ordinate indicates the A230 nm (arbitrary unit of HPLC UV-detector) and NaCl concentration (mM).