Heparin and non-anticoagulant heparin attenuate histone-induced inflammatory responses in whole blood
Fig 2
Effect of heparin on background and histone induced inflammatory responses in whole blood.
Increasing concentrations of heparin (in International Units) were added to whole blood treated with/without 50 μg/ml histones and incubated for 6 hours. Released C3a, IL-6 and IL-8 were measured in plasma and tissue factor was measured from the cellular component. Analysis of each group (with/without histones) by Dunnett’s multiple comparison was carried out using the background (0 IU) as the control group, * or # = p < 0.05, ** or ## = p < 0.01, *** or ### = p <0.001. Error bars = standard deviation of the average from 6 donors.