The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research
Fig 6
Other applications of fasudil.
A: Supportive effect of fasudil on hES3 survival and growth in suspension culture. The fasudil-treated groups and Y-27632-treated groups were similar in the rate of formation of cell aggregates and their size; the control cells did not form aggregates. Scale bar = 200 μm (*: p < 0.05, 2 biological replicates, 7 technical replicates). B: Seven-day cultures of single hES3 cells on vitronectin-coated culture plates in the presence of 10 μM Y-27632 or fasudil. Scale Bar = 200 μm. The growth of hPSCs was faster with Y-27632 or fasudil than in the untreated control (*: p < 0.05, 3 technical replicates). hES: human embryonic stem cell, hPSC: human pluripotent stem cell.