The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research
Fig 5
Fasudil promotes differentiation of PSCs.
A: Expression of RPE-specific markers; PAX6 and MITF examined by immunocytochemistry (ICC) at 13 days. B: PAX6 and MITF positive cell populations analyzed by flow cytometry at 13 days. The percentage of PAX6 was similar in the groups, while MITF-positive cells were more frequent in the treated groups than in the untreated control (*: p < 0.05, 3 replicates). Fasudil did not affect cell number in RPE differentiation. C: The expression of NCC markers; PAX3 and SOX10 by ICC. D: PAX3 and SOX10 positive cell population analysis using flow cytometry at 6 days. Fasudil promoted cell survival during NCC differentiation of hiPSCs (*: p < 0.05, 3 replicates). RPE: retinal pigment epithelium, NCC: neural crest cell.