INCB054828 (pemigatinib), a potent and selective inhibitor of fibroblast growth factor receptors 1, 2, and 3, displays activity against genetically defined tumor models
Fig 2
INCB054828 inhibits FGFR-dependent signaling pathways.
(A) KG1a or (B) RT-4 cells were treated with INCB054828 for 2 hours, lysed and subjected to immunoblotting for phospho- and total proteins in the FGFR signal transduction pathway including FGFR, ERK, FRS2, and STAT5. (C) Concentration-dependent inhibition of phospho-FGFR3 by INCB054828 in RT-112 cells was determined using a proximity ligation assay with a mouse monoclonal anti-phospho-FGFR (Y653/Y654) and rabbit anti-FGFR. Original Western blot images are shown in S1 File (S1 Raw images).