Biochemical characterization of the Nocardia lactamdurans ACV synthetase
Fig 6
Overexpression and activity assays of the hybrid NRPSs.
(A) SDS-PAGE analysis of the purified fractions of the hybrids: only fraction E2 (150 mM imidazole) is shown. Marker lane shows reference protein corresponding to 180 kDa. (B) In vitro tripeptide production assays with hybrid enzymes. (C) The production of the tripeptide ACV was confirmed by accurate monoisotopic mass and equal retention time compared to the wild-type enzyme product and an ACV synthetic standard.