Biochemical characterization of the Nocardia lactamdurans ACV synthetase
Fig 3
Enzymatic characterization of ACVS.
Three reaction series were conducted using various concentrations of L-α-Aaa (A), L-Cys (B) and L-Val (C) and analyzed by LC/MS to quantify the amounts of the ACV tripeptide produced for Michaelis-Menten kinetics (D).