Incompatible erythrocyte transfusion with lipopolysaccharide induces acute lung injury in a novel rat model
Fig 5
LPS+30% transfusion increases neutrophil-mediated lung injury.
Blinded grading of H&E sections for neutrophil infiltration and cell wall thickening from sham (n = 20) animals and animals receiving 15 (n = 15), 30 (n = 30), 45% (n = 15) human erythrocyte transfusion (xt), LPS alone (n = 15) and LPS+30% transfusion (n = 20). Tissues were scored on a scale of 0–4: 0 = normal lungs, 1 = minor lung involvement, 2 = moderate lung involvement, 3 = serious lung involvement, 4 = severe lung involvement. Data are means and standard error of the mean. Statistical analysis was performed using a Kruskal-Wallis test, followed by Mann-Whitney test. * denotes P = 0.05 compared to all other experimental groups.