Intestinal enteroids recapitulate the effects of short-chain fatty acids on the intestinal epithelium
Fig 7
(A) Alcian blue/Hematoxylin staining 2D enteroids treated with 10 mM acetate (ACET), butyrate (BUT), and propionate (PROP) compared to controls (CON). (B) Zonula occludens-1 (ZO-1) staining (red) and (C) Claudin-3 (CLDN3) staining (red). Nuclei are stained with DAPI (blue). (D) Western blot data for Chromogranin-A (CHGA), Zonula-occludens-1 (ZO-1), occludin (OCLN), and Trefoil Factor 3 (TFF3). Samples were compared to β-actin as a housekeeping protein. Confocal images are representative of several fields of view at 40X magnification. Treatments are compared to controls (CON) which are set to 1.0 and superscripts (a,b,c,d) indicate statistical significance between treatments at P < 0.05, n = 4.