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Intestinal enteroids recapitulate the effects of short-chain fatty acids on the intestinal epithelium

Fig 5

Effect of 24 h treatment with 10 mM acetate (ACET), butyrate (BUT), or propionate (PROP) on 3D (a) and 2D (b) human small intestinal enteroid cell proliferation (EdU staining, green) compared to controls (CON).

Nuclei are stained with DAPI (blue) and images are representative of several fields of view at 20X magnification. Treatments are compared to controls (CON) which are set to 1.0 and superscripts (a,b,c,d) indicate statistical significance between treatments at P < 0.05, n = 6.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0230231.g005