Intestinal enteroids recapitulate the effects of short-chain fatty acids on the intestinal epithelium
Fig 4
Effect of 24 h treatment with acetate (ACET), butyrate (BUT), or propionate (PROP) at (A) 1 mM and (B) 10 mM.
Gene expression of differentiation markers: stem cells (Lgr5), enterocyte alkaline phosphatase (Alpi), goblet cell mucin 2 (Muc2), enteroendocrine cell chromogranin A (Chga), Paneth cell lysozyme (Lyz), tight junction markers claudin-3 (Cldn3), occludin (Ocln), zonula-occludens 1 (Tjp1), Tuft cell marker Dclk1, anti-microbial peptide expression Reg3β, Reg3γ, and beta-defensin 1 (Defb1) were analyzed in human 3D duodenal enteroids. Treatments are compared to controls (CON) which are set to 1.0 and superscripts (a,b,c,d) indicate statistical significance between treatments at P < 0.05, n = 4.