NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization
Fig 4
Two-step workflow for the identification of thirteen different organisms by DOPE-FISH.
Probes that were designed and evaluated in this study are underlined in black. Note that B. mallei is detected by the probes Bmal and Bboth, while B. pseudomallei is detected by Bboth only. F. tularensis belongs to the Gammaproteobacteria, but is detected by the probe Bet42a. Bacterial FISH images are drawn to scale and represent 10 μm squares each.