NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization
Fig 2
Influence of lyophilization and prolonged storage on the probe signal intensity in urea-based hybridizations.
The Cy3-labeled Gam42a, Cy5-labeled Bet42a and FLUOS-labeled EUB probes were lyophilized and used in hybridizations either directly after lyophilization or after storage at room temperature for 28 days. Signal intensities were compared to signal intensities of freshly prepared reagents without lyophilization. A ratio of 1 means no change, while ratios below 1 indicate negative influence of lyophilization or storage. E. coli was used as target organism for Gam42a, N. meningitidis for Bet42a and both organisms as target for the EUB probe. The means and the standard deviation based on at least three replicates are shown.