Assessment of acyl-CoA cholesterol acyltransferase (ACAT-1) role in ovarian cancer progression—An in vitro study
Fig 9
Effect of shRNA mediated ACAT-1 inhibition on reactive oxygen species (ROS).
Detection of ROS was made by flow cytometry. Data were presented as relative-fluorescence intensity in a 2-dimensional FACS profile (standardized gating, 20,000 events). Enhanced ROS generation is observed as a shift the in peaks in ACAT-1 inhibited cell lines (blue) compared to scrambled controls (red). All experiments were performed in triplicate.