Assessment of acyl-CoA cholesterol acyltransferase (ACAT-1) role in ovarian cancer progression—An in vitro study
Fig 5
Effect of shRNA mediated ACAT-1 inhibition on cell cycle.
(A): Cell cycle analysis of ovarian cancer cell lines stably transfected with control shRNA or ACAT-1 shRNA. Arrows (Ap) represent sub-G1 (apoptotic) phase. (B): Graphical representation of cell cycle analysis. Data are presented as mean ± SD of triplicate experiments. *P < 0.05, compared to respective scrambled controls, Student’s t-test. (C) Effect of ACAT-1 inhibition on apoptosis. Nuclear condensation indicative of apoptosis was assessed using NucBlue Live Cell Stain Hoechst 33342. Representative images were taken with an inverted microscope (Olympus H4-100, CCD camera) and 20× objective.