Assessment of acyl-CoA cholesterol acyltransferase (ACAT-1) role in ovarian cancer progression—An in vitro study
Fig 3
Effect of ACAT-1 inhibition on viability of a panel of ovarian cancer cell lines.
(A) Cell viability assay of a panel of ovarian cancer cell lines treated with ACAT inhibitor avasimibe for 3 days. Vehicle treated cells (media with 1% DMSO) were considered as control against which treated cells were compared. Data expressed as mean ± SD of triplicate experiments. *P < 0.05, compared to control, Student’s t-test. (B) Confirmation of efficient stable ACAT-1 knock down assessed by western blot. β-actin was used as the loading control. (C) Assessment of cell viability upon stable knock-down of ACAT-1, in a panel of ovarian cancer cell lines. Data are expressed as mean ± SD of triplicate experiments. *P < 0.05, compared to the respective scrambled controls, Student’s t-test.