Genomic structure and transcript analysis of the Rapid Alkalinization Factor (RALF) gene family during host-pathogen crosstalk in Fragaria vesca and Fragaria x ananassa strawberry
Fig 1
Fragaria x ananassa (Fxa) RALF genes phylogentic analysis, evolution and chromosome organization.
(a) Phylogenetic tree was built aligning 50 FanRALF protein sequences using MUSCLE. The tree is drawn to scale, with branch lengths measured in the number of substitutions per amino acidic site in the protein sequences. Gene annotations refer to F. × ananassa cv. Camarosa v1.0.a1 and are listed in S1 Table. Progenitor lineage were inferred from gene chromosome location (b), and pink was used for F. vesca subgenome, green for F. nipponica, light blue for F. viridis and orange for F. iinumae. The color legend also reports the code number used to name different FanRALF genes according to subgenome lineage, as is reported in S1 Table. (b) FanRALF genes chromosome spatial organization in the octoploid genome. (c) Pie chart showing total FanRALF gene members present in the four subgenomes.