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Phosphorylation-dependent activity-based conformational changes in P21-activated kinase family members and screening of novel ATP competitive inhibitors

Fig 1

Comparative sequence and structural analysis.

(A) Multiple sequence aligment of group-I. (B) Multiple sequence aligment of group-II. (C) Superposed ribbon diagram PAKs group-I members. (D) Superposed ribbon diagram PAKs group-II members. Secondary structure is delineated at the upper side of sequence as β-strands (arrows) and α-helices (coil). ATP-binding residue (β3) Lys, (αC) Glu, (β5) Glu and (β8) Lys are delineated in navy blue, brown, khaki, magenta and aqumarine colors, respectively. Activation loop (T-loop) and phosphorylated residues (T/S) are delineated in lime green and sandy brown colors. N- and C-lobes are indicated in light blue and white colors, respectively.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0225132.g001