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Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis

Fig 2

SGLT1 staining in lung tissues.

Alveolar structures in lung from Sham (A to D), CLP saline (CLP-sal, E to H), CLP salbutamol (CLP-salb, I to L) and CLP phlorizin (CLP-phlo, M to P) treated rats. Sections (A, E, I and M) were immunostained with anti-F-actin antibody (red). White enclosed boxes showing an alveolar septum and alveolar lumen, taken with a greater resolution, are presented in the next sections: (B, F, J and N) F-Actin (red), (C, G, K and O) SGLT1 (green) and (D, H, L and P) merged photomicrographs for co-localization of SGLT1 and F-actin (yellow to orange). White arrows indicate the presence of SGLT1 in the luminal membrane. White asterisks indicate SGLT1 in cytoplasm. Description: (AL) alveolar lumen of the rat pulmonary section. Magnification, x600, scale bar, 10 μm. Section Q and R show the protein level in cytoplasm or plasma membrane, respectively. Images are representative of 4 animals in each group.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0222575.g002