Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts
Fig 6
Fructose-1,6-bisphosphate regulates expression of ECM and ECM-degrading proteins in vitro.
(A) qRT-PCR analysis for the levels of Fn1, Cxcl12, Cdh1, superoxide dismutase 1 (Sod1), and metalloproteinase 2 (Mmp2) mRNAs. Relative expression is presented based on the expression level of control as 1. One-way ANOVA followed by Tukey’s multiple comparison test was used. Values are expressed as mean ± SD; n = 4–6/group; *p<0.05; **p<0.01; ***p<0.001. (B) Protein expression of α-SMA, COL1A1, MMP1, MMP2, TIMP-1, and TIMP-4 in HLF and FLF treated with FBP for 3 days.