Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
Fig 8
Blastocyst development and quality of juvenile-goat oocytes after biphasic IVM (6 h pre-IVM with CNP plus E2, followed by 24 h IVM) and control IVM (24 h).
(A) Cleavage rate (48 hpf) and blastocyst rate (8 dpf) per number of oocytes (N). A total of 196–198 oocytes were cultured per group (5 replicates). (B) Cell count of the inner cell mass and the trophectoderm of expanded and hatched blastocysts. A total of 22–33 blastocysts were analyzed per group (4 replicates). Each bar represents mean + SEM. Different superscript letters (a-b) in each column indicate statistically significant differences (P<0.01).