Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
Fig 6
Effect of biphasic IVM on GSH and ROS levels of juvenile-goat oocytes.
(A) H2DCF-DA-ROS average fluorescence intensity per oocyte after biphasic IVM (6 h pre-IVM with CNP and E2, followed by 24 h IVM) and control IVM (24 h) and representative images. (B) MCB-GSH average fluorescence intensity per oocyte following biphasic and control IVM and representative images. A total of 30 oocytes were assessed per group (3 replicates). Each bar represents mean + SEM. Different superscript letters (a, b) in each column indicate statistically significant differences (P<0.01).