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Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes

Fig 4

Nuclear maturation rate of juvenile-goat oocytes after biphasic IVM (6 h pre-IVM with CNP and estradiol, followed by 24 h IVM) and control IVM (24 h).

Nuclear stage was classified as GV: germinal vesicle; MI: metaphase I; MII: metaphase II. A total of 40 oocytes were assessed per group (4 replicates). Each bar represents mean + SEM.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0221663.g004