Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
Fig 3
Effect of pre-IVM with CNP and estradiol on the transzonal projections (TZPs) density of juvenile-goat COCs.
(A) Phalloidin-FITC Average fluorescence intensity in the zona area of COCs after recovery (control 0 h), 6 h of IVM, 6 h of pre-IVM with CNP and E2, 24 h of IVM, and 6 h of pre-IVM followed by 24 h IVM. At least 32 COCs were assessed per group (4 replicates). Each bar represents mean + SEM. Different superscript letters (a-c) in each column indicate statistically significant differences (P<0.05). (B) Representative confocal images. Positive actin filaments are observed as continuous filaments going from the cumulus cells to the oocyte through the zona region.