Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
Fig 2
Effect of pre-IVM with CNP and estradiol for 6 h on germinal vesicle chromatin configuration of juvenile-goat oocytes.
Germinal vesicles (GV) are classified as GV1: diffuse filamentous chromatin; GV net-like: condensed net-like chromatin; GV clumped: condensed clumped chromatin; GVBD: broken nuclear membrane. A total of 46–50 oocytes per treatment were assessed (4 replicates). Each bar represents mean + SEM. Different superscript letters (a-c) in each column indicate statistically significant differences (P<0.05).