Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures
Fig 8
Transcript level of BCRP, MRP-1 and P-gp and cell localization of P-gp in endothelial cells before, during and after 4 h of oxygen-glucose deprivation (OGD) or medium exchange.
The mRNA levels of BCRP (A), MRP-1 (B) and P-gp (C) at the different time points of the OGD (black bars) and medium exchange (gray bars) protocol were measured by RT-qPCR and normalized to reference genes. Primers specifically designed for bovine orthologues were utilized. Bar graphs represent means and error bars are +SEM. N = 3, n = 3–4. Columns were compared to t0 using one-way ANOVA and Dunnett’s multiple comparison post-test. *: p<0.05, **: p<0.01, ***: p<0.001. Bonferroni’s post-test was utilized to compare each pair of columns. #: p<0.05. Figs D-J show antibody staining of P-gp (green), and cell nuclei staining with propidium iodide (red) under the different treatments. Figs D’-J’ show exclusively the P-gp staining from Figs D-J. Bars = 10 μm. N = 1; n = 3.