Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures
Fig 6
Transcript levels of membrane transporter GLUT-1 and the receptor HB-EGF, InsR, TfR, LDLR and LRP-1 in endothelial cell monolayers before, during and after oxygen-glucose deprivation (OGD) treatment or medium exchange protocol.
The transcript level of GLUT-1 (A) and different receptor proteins (B-F) in endothelial cells that had undergone OGD (black bars) or medium exchange (gray bars) were evaluated by RT-qPCR and normalized to reference genes. Primers specifically designed for bovine orthologues were utilized. Bar graphs represent means and error bars are +SEM. N = 3, n = 3–4. Columns were compared to t0 using one-way ANOVA and Dunnett’s multiple comparison post-test. *: p<0.05, **: p<0.01, ***: p<0.001. Bonferroni’s post-test was utilized to compare each pair of columns.