Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures
Fig 4
Cellular localization of ZO-2 along the oxygen-glucose deprivation (OGD) and medium exchange protocols.
Endothelial cells in co-culture with astrocytes treated with OGD or medium exchange and reperfused for 24/48 h, were fixed and stained with an antibody anti-ZO-2. Fig A shows the localization of ZO-2 before the treatments (t0), Fig B shows the localization of ZO-2 after 4 h from medium exchange, and Fig C shows the localization of ZO-2, 4 h after OGD. Bars = 10 μm. N = 1; n = 3. (D) For each condition, the intracellular signal intensity was estimated using ImageJ as described in the “Materials and methods” section. Bar graphs represent means normalized to t0 and error bars are +SEM. (N = 9–12, n = 3–4). The white bar shows the value at t0, the gray bars show the cells subjected to medium exchange, while the black bars show the OGD treated cells. Columns were compared to t0 using one-way ANOVA and Dunnett’s multiple comparison post-test. *: p<0.05, ***: p<0.001. Bonferroni’s post-test was utilized to compare each pair of columns. #: p<0.05.