A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species
Fig 5
Pv33 induces cell death in Nicotiana spp.
Agrobacterium-mediated transient expression of Pv18, Pv31, Pv33, Pv82 without their signal peptide (Δsp) (A) and full-length Pv33, including its signal peptide (FL) (B) in N. benthamiana, N. tabacum and N. occidentalis leaves. All pictures were taken 5 days after agroinfiltration. (C) Constructs used in experiments shown in D and E. PR1SP: signal peptide of PR1. Pv33SP: signal peptide of Pv33. (D and E) Confocal images following infiltration of N. benthamiana leaves with the constructs described in C, without (D) and with (E) BFA treatment. Red arrows show nucleus. Bars show 10 Δm. In A and B, for each species, 6 leaves were infiltrated with all the effectors. Experiments were repeated twice with the same results.