Detection of the KIAA1549-BRAF fusion gene in cells forming microvascular proliferations in pilocytic astrocytoma
Fig 5
FISH analysis for the detection of the KIAA-BRAF fusion gene.
(A) FISH probes at the duplication site of the BRAF gene (7q34) are labeled with Texas red. The centromere of chromosome 7q (7q11.21) is labeled with fluorescein isothiocyanate (FITC, green). Due to tandem duplication at 7q34 leading to the expression of the KIAA-BRAF fusion gene, cells with the fusion gene demonstrated gain of a red signal (total: three signals) in comparison with the green signal (two signals). (B) In both S1 and S6, tumor cells and cellular components of MVP were positive for the KIAA-BRAF fusion gene.