Isolation, cloning and expression of CCA1 gene in transgenic progeny plants of Japonica rice exhibiting altered morphological traits
Fig 5
PCR analysis of T0, T1 and T2 transgenic plants for the presence of the hyg gene.
Arrowhead indicates the 324 bp band corresponding to the hyg gene of the T-DNA. The pCAMBIA1300 served as a Positive Control (+ve) and non-transgenic Taipei 309 served as a Negative Control (–ve). (A) PCR Analysis of T0 Transgenic Lane 1 L—1 kb ladder, lane 2- (–ve control, lane 3 +ve control, lanes 4–8—putative transgenic plants of construct A, lanes 9–13- putative transgenic plants of construct B, lanes 14–18 putative transgenic plants of construct C, lane 19–1 kb ladder. (B) PCR Analysis of T1 Transgenic Plants. Lane 1- L—1 kb ladder, lane 2 –ve control, lanes 3–4 putative transgenic plants of construct A, lanes 5–9- putative transgenic plants of construct B, lanes 10—putative transgenic plants of construct C, lane 11 +ve control. (C) PCR Analysis of T2 Transgenic Plants. Lane 1- L—1 kb ladder, lane 2–9 putative transgenic plants of construct A, lanes 10–16 putative transgenic plants of construct B. (D) PCR Analysis of T2Transgenic Plants. Lane 1- L—1 kb ladder, lanes 2–12 putative transgenic plants of construct B, lanes 13–16 putative transgenic plants of construct C, lane 17 +ve control. The gel images for Fig 5A, 5C and 5D is original. The gel images for Fig 5B have been cropped; full length gel pictures have been shown as S7 Fig.