A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a
Fig 5
Detection of the transcription of a fluorescent aptamer.
(a) Scheme of the transcription of iSpinach aptamer, which binds the fluorophore DFHBI and increases its fluorescence. (b) Measurement of the concentration of iSpinach:DFHBI complex during transcription on the detector and in bulk (inset), with various concentrations of DNA template: 0 nM (grey), 25 nM (light blue), 50 nM (median blue) and 100 nM (dark blue). Thick line and shaded area represent respectively mean and measurement uncertainty as computed in S1 Appendix.