A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a
Fig 4
(a) The user interface of the software allows to perform a calibration (to determine the dependence of the measured resistance on fluorophore concentration) or directly measure a sample. (b) Several parameters can be adjusted, such as the calibration parameters and the frequency of data acquisition. c) During measurements, acquired data can be plotted in real time.