A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a
Fig 3
Plastic filter foils for green fluorescence detection and calibration procedure.
(a) Excitation and (b) emission spectra of fluorescein (green) overlaid with the corresponding filter foil transmission spectra. The grey area in a) indicates the interval spanning 95% of the LED intensity. The combination of two blue and one orange filter foils allows for sufficient transmission of the excitation and emission light respectively, while blocking nearly all bleed-through excitation. (c) Calibration of the measured relative resistance for different dilutions of fluorescein over 3 replicates and 2 measurements per replicate. (d) Relative measurement uncertainty calculated as a function of the fluorescein concentration. The confident measurement range is taken as the range where the relative uncertainty is below 15%.