A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling
Fig 2
GenBank taxonomies of reference-free tags for ApeKI (a) and PstI (b). Tags were compared against the GenBank database using BLAST and taxonomy was assigned using the MEGAN algorithm considering only hits with the top bitscore for that tag. This figure shows the taxonomy of tags at the kingdom level, and within bacteria and eukaryota at the phylum level and within archaea at the class level. Graphs show the proportion of tags assigned to each taxonomic level and do not reflect the relative abundance of each tag.