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Antibody conjugation to carboxyl-modified microspheres through N-hydroxysuccinimide chemistry for automated immunoassay applications: A general procedure

Fig 5

Relative fluorescence intensity (%) versus mass of anti-CBZ IgG-Cy5 (μg) added to 500 μg of TentaGel beads.

Data were obtained from two independent runs (run one red square, run two blue dots, 20 h activation, 240 min coupling). Linear Least-Squares fit yielded a slope of 8.9% RFI μg-1 and an intercept of 13% RFI (R2 = 0.983) for Run 1, and a slope of 8.5% RFI μg-1 and an intercept of 15% RFI (R2 = 0.990).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0218686.g005