Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle
Fig 4
Cyclin amounts in different conditions and stresses.
Strains were grown and processed as described in Fig 2B. Western blots were quantified, values were standardized using loading control, relativized to the maximum expression amounts and plotted as in Figs 1B and 3B. A) Cyclin amounts in particular environmental or stress conditions were obtained by calculating the area under the curve from α-factor release to START. B) Pcl and Cln amounts were calculated as for A). Conditions are ordered according to increasing amounts of Pcl. C) Same procedure as in A) but with the different members of the two cyclin families separated. Conditions are ordered according to increasing amounts of Pcl. Values are expressed as means of at least three independent experiments.