MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1
Fig 2
Overexpression of miR-582-5p inhibits proliferation and invasion of NCI-H358 cell.
(A) NCI-H358 cells were transfected with miR-582-5p mimics and then miR-582-5p expression was examined with qPCR assay. (B) After transfection with miR-582-5p mimics, NCI-H358 cell proliferation was measured by using CCK-8 assay at different times (0 h, 24 h, 48 h and 72 h). (C) Western blot assay was performed to determine the expression of E-cadherin and Vimentin. (D) Relative expression of E-cadherin and Vimentin was quantified by using Image J software and GraphPad Prism 5.0. (E) Transwell assay was conducted to evaluate the invasive potential of NCI-H358 cells. (F) Relative invasive cells were estimated with Image-Pro Plus tool and GraphPad Prism 5.0. Data are showed as the mean ± SD (n = 3). *P<0.05, **P<0.01 and **P<0.001 versus the scramble group.