An advanced recording and analysis system for the differentiation of guinea pig cough responses to citric acid and prostaglandin E2 in real time
Fig 2
A screen shot showing our experimental recordings and analyses in real-time in a conscious guinea pig exposed to aerosolized CA.
The symbols (a)—(d) represent four windows in the screen. On the left side, the real-time video monitoring of animal body posture and the list of comments are presented in a- and b-windows, respectively. The channels (Ch) on the right side from the top to bottom are spectrogram (Ch1 in c-window), respiratory flow, sound, accumulated sneeze count/sneeze marker (SM), accumulated cough count/cough marker (CM), cough sound integrated intensity (CSII), cough sound duration (CSD), and respiratory frequency (fR) (Ch2 through Ch8 in d-window). In real-time, SM and CM are manually marked by symbol “*”, while the accumulated sneeze and cough count, CSII, CSD, and fR are automatically calculated and displayed on the screen. Note: the time-scale in c-window is set to 40-fold smaller than that in d-window for a better visual assessment of the cough-related spectrogram. Therefore, the cough spectrogram in c-window is corresponding to the last cough in d-window. The numbers with CM “*” in Ch5 as enlarged in the inset is the comment numbers but not the cough numbers. There are three columns in d-window: the scale and unit of the variable in each channel (left), the signals for each channel (middle), and the name and the value of each channel in real time (right). The lines in Ch5, 6 and 7 (the middle column) present the levels of cough numbers, intensity, and duration of individual coughs, while the values of the accumulated cough numbers, the cough intensity and duration of the last cough are displayed in Ch5, 6 and 7, respectively, of the right column.