High-throughput identification of heavy metal binding proteins from the byssus of chinese green mussel (Perna viridis) by combination of transcriptome and proteome sequencing
Fig 1
Strategy for integration of transcriptome and proteome sequencing.
(a) The foot area, byssal threads and byssal plaques (rectangles from bottom to top) were dissected for sequencing. (b) Transcriptome sequencing of the foot area was performed for subsequent de novo assembly and annotation. (c) Thread and plaque proteins were separated by SDS-PAGE before LC-MS/MS analysis. (d) The generated transcriptome data were integrated with the proteome sequencing data to identify interesting transcripts and deduce their corresponding protein sequences. Further protein structural analysis, recombinant protein engineering, and biomimetic material processing are examples of potential applications.